Edinburgh flanker Luke Crosbie will captain Scotland A against Chile in Santiago on Saturday.
The game kicks off a South America tour that will see the senior Scotland team tackling Argentina in a three-Test series next month.
Crosbie made his Scotland debut against Tonga last year and he leads a side that includes experienced internationals like scrum-half George Horne, prop Jamie Bhatti, lock Scott Cummings and number eight Matt Fagerson.
Cummings missed this season’s Six Nations through injury and he is partnered in the second-row by Edinburgh’s Jamie Hodgson.
Scotland’s Gallagher Premiership-based players are unavailable for the game as it falls outside World Rugby’s international fixture window.
Chile will use the match as preparation for their World Cup play-offs against the United States.
Scotland A have not played since February 2014, when they drew 16-16 against England Saxons in Glasgow.
Scotland A team to play Chile
Forwards: J Bhatti (Glasgow), D Cherry (Edinburgh), J Sebastian (Scarlets), J Hodgson (Edinburgh), S Cummings (Glasgow), B Muncaster (Edinburgh), L Crosbie (Edinburgh, CAPTAIN), M Fagerson (Glasgow)
Backs: G Horne (Glasgow), R Thompson (Glasgow), R McLean (Glasgow), S Tuipulotu (Glasgow), M Currie (Edinburgh), D Hoyland (Edinburgh), O Smith (Glasgow)
Replacements: J Matthews (Glasgow), P Schoeman (Edinburgh), M Walker (Glasgow), G Young (Edinburgh), M Bradbury (Edinburgh), A Price (Glasgow), B Kinghorn (Edinburgh), M Bennett (Edinburgh).